In vitro ectomycorrhizal formation of Pinus patula, P. pseudostrobus, P. oocarpa and P. elliotii grown in southern India     

M. Sudhakara Reddy  K. Natarajan 《New Forests》1996,11(2):149-153

In vitro syntheses of ectomycorrhizas of Pinus patula, P. pseudostrobus, P. oocarpa and P. elliotii with Amanita muscaria, Laccaria fraterna, L. laccata, Pisolithus tinctorius, Rhizopogon luteolus, Scleroderma citrinum and Thelephora terrestris were attempted. Most of the fungi initiated mycorrhizal formation within 2 weeks after inoculation. Laccaria fraterna and S. citrinum formed mycorrhizas after 6 weeks of inoculation. Amanita muscaria failed to form mycorrhizas during the 12 week period of observation. The degree of colonization of different mycorrhizal fungi varied with different species of Pinus.  相似文献   

Prevalence of nematode parasite,Contracaecum, in Nile tilapia,African catfish and Barbus species in Lake Hawassa,Ethiopia     

Zinabu Tesfaye  Beshatu Ferede  Natarajan Pavanasam  Kassaye Balkew Workagegn 《Aquaculture Research》2020,51(10):3993-3998

A cross‐sectional study was conducted from November 2017 to March 2018 at Lake Hawassa to determine the prevalence of larval Contracaecum infestation in Nile tilapia, (Oreochromis niloticus), African Catfish (Clarias gariepinus) and Barbus species (Barbus intermedius). Fifty‐two point six per cent (52.6%) previous prevalence and 5% precision were used to estimate the sample size. Accordingly, a total of 383 randomly sampled fish species comprising of 163 (42.6%) C. gariepinus, 159 (41.5%) O. niloticus and 61(15.9%) B. intermedius were examined. The length and weight of each sampled fish were recorded. All the sampled fish were examined for the evidence of Contracaecum parasites in Hawassa University Veterinary Parasitology and Pathology Laboratory. Chi‐square values and comparison of proportions were used to analyse the data. The overall prevalence of Contracaecum parasites of fish population was 31.6%. The distribution of parasite was significantly affected by fish species (p = .000), sexes (p = .018), length (p = .003) and weight classes of fish (p = .026). As a hygienic problem and gutting activity conducted at the sides of the Lake Hawassa and distribution of discarded fish wastes for surrounding piscivorous birds by butchers and other people, the life cycle of Contracaecum parasite was perpetuated. Thus, the parasite is of zoonotic significance. Therefore, awareness creation activities for societies and control of fish parasites should be conducted in the study area.  相似文献   

Limited contribution of water availability in genotype-by-environment interaction in sugarcane yield and yield components     

Sijesh Natarajan  Jaya Basnayake  Prakash Lakshmanan  Shu Fukai 《Journal of Agronomy and Crop Science》2020,206(6):665-678

Water deficit is an important abiotic stress factor limiting sugarcane production. Understanding the effect of water deficit on cane yield, yield components and genotype × environment (G × E) interaction can aid in selecting genotypes that are adapted to water deficit conditions. In this study, response of cane yield and yield components of 10 sugarcane genotypes grown under 3 irrigation treatments—fully irrigated, semi-irrigated and rainfed—was examined across 3 locations and 3 crop-years within each location. Cane yield reduced by 32% and 9% in the rainfed and semi-irrigated treatments, respectively, compared to the fully irrigated treatment. Genotypic variation was highly significant for cane yield and yield components in all three irrigation regimes. Cane yield in the rainfed treatment showed significant genetic correlation with stalk diameter (r_g = 0.68), leaf number (r_g = 0.64), node number (r_g = 0.67) and stalk number (r_g = 0.68). Genotype × irrigation variance was negligible compared to genotype, genotype × location and genotype × crop-year variances. Commercial genotypes had higher yield in most environments except in low-yield potential environments, which caused a significant genotype × location × irrigation effect, suggesting opportunities for improving sugarcane productivity in these environments.  相似文献   

Imbibition of soybean seeds in warm water results in the release of copious amounts of Bowman-Birk protease inhibitor, a putative anticarcinogenic agent     

Palavalli MH  Natarajan SS  Wang TT  Krishnan HB 《Journal of agricultural and food chemistry》2012,60(12):3135-3143

Protease inhibitors play a protective role against pathogenic microorganisms and herbivorous insects. The two predominant protease inhibitors of soybean seeds are the Kunitz trypsin inhibitor (KTI) and Bowman-Birk protease inhibitor (BBI). In this study, we report that soybean seeds incubated in warm water release large amounts of proteins into the surrounding media. Two-dimensional gel electrophoresis analysis of the seed exudates resulted in the separation of 93 distinct protein spots out of which 90 spots were identified by LC-MS/MS. The basic 7S globulin and the BBI are the two predominant proteins found in the soybean seed exudates. In addition to 7S and 11S seed storage proteins, others known to protect the seeds against pathogens and pests including KTI, peroxidase, α-galactosidase, and endo-1.3-β-glucanase were also identified in the seed exudates. Soybean seed exudate obtained by incubating the seeds in warm water was also able to inhibit the growth of human breast cancer cell line MCF-7. Since soybean seeds release large amounts of enzymatically active BBI when immersed in warm water, our procedure could be exploited as a simplified alternative method for the preparation of BBI concentrate which is being used as a cancer chemoprotective agent.  相似文献   

Assessment of genetic relatedness among mango cultivars of India using RAPD markers     

K. V. Ravishankar  Lalitha Anand  M. R. Dinesh 《The Journal of Horticultural Science and Biotechnology》2013,88(2):198-201

Summary

DNA-based RAPD (Random Amplification of Polymorphic DNA) markers have been used extensively to study genetic relationships in a number of fruit crops. A wide genetic diversity exists in the mango fruit in India. Present day commercial cultivars originated mainly from this subcontinent. In this study, 18 commercial mango cultivars, traditionally grown in western, southern, northern and eastern parts of India, were selected to assess genetic relatedness. Total genomic DNA was extracted and subjected to RAPD analysis using 30 arbitrary 10-mer primers. Of these, 27 primers amplified mango genomic DNA. None of these primers produced unique band pattern for each cultivar. RAPD data were used to calculate a squared Euclidean distance matrix, and based on this cluster analysis was done using a minimum variance algorithm. Cluster analysis clearly showed two groups—the first consisting of western, northern and eastern mango cultivars and the second group consisting of southern cultivars. From the analysis of results, it appears the majority of mango cultivars originated from a local mango genepool and were domesticated later.  相似文献   

Analysis of genetic diversity among Indian short-day onion (Allium cepa L.) cultivars using RAPD markers.     

M. Sangeeta Kutty  Lalitha Anand 《The Journal of Horticultural Science and Biotechnology》2013,88(4):774-778

Summary

Randomly amplified polymorphic DNA (RAPD) markers were used to estimate genetic diversity among 24 cultivars of short-day onions. Total genomic DNA was extracted and subjected to RAPD analysis using 90 arbitrary 10-mer primers. Of these, 15 primers were selected which yielded 137 bands, 91.24% of which were polymorphic. None of the primers produced a unique banding pattern for each cultivar. RAPD data were used to calculate a Squared-Euclidian Distance matrix which revealed a minimum genetic distance between cultivars ‘AFLR-722’ and ‘PBR-140’, and a maximum genetic distance between cultivars ‘PBR-139’ and ‘A.Kalyan’, and ‘MS-48’ and ‘A.Kalyan’. Based on the distance matrix, cluster analysis was done using a minimum variance algorithm.The dendrogram thus generated, based on Ward’s method, grouped the 24 onion cultivars into two major clusters. The first cluster consisted of cultivars from the northern region, and the second of cultivars from the southern region of India. The present study shows that there is high diversity among the onion cultivars selected and indicates the potential of RAPD markers for identification and maintenance of onion germplasm for crop improvement purposes.  相似文献   

Biophysical and socio‐economic causes for increasing fallow land in Tamil Nadu          下载免费PDF全文

S. Dharumarajan  M. Lalitha  A. Natarajan  L. G. K. Naidu  R. Balasubramanian  R. Hegde  R. Vasundhara  K. S. Anil Kumar  S. K. Singh 《Soil Use and Management》2017,33(3):487-498

The question of increasing fallow land assumes significance at national and international levels due to the decline in net cultivated area and increasing population. Biophysical characteristics of farmland and socio‐economic conditions of farmers are responsible for the increasing fallow. This study was carried out to assess the extent of permanent fallow and identify causes for its increase. High‐resolution remote sensing data were used to identify fallow land in two identified blocks of south India, viz. Nanguneri and Kangeyam. The land resources were characterized, and the specific biophysical and socio‐economic factors responsible for the increasing fallow land were assessed. Multiple regression analysis was carried out to identify the factors responsible for increasing fallows. Soil parameters such as erosion, soil texture, soil depth and available nitrogen contributed significantly (P > 0.05) to the increase in fallow land. The size of farm holding, number of farm fragments, distance from the road in Nanguneri and farm size and the distance to the nearest town in Kangeyam were socio‐economic factors that had a strong effect (P > 0.01) on increasing the fallow land. Variable importance rankings in random forest analysis indicated that soil erosion and soil pH had the largest impact on the decision to fallow land in Nanguneri and Kangeyam blocks, respectively. Based on biophysical and socio‐economic constraints, the land resources of fallow land were evaluated for other alternate land use options and policy measures were suggested.  相似文献   

Viral infections in cultured fish and shrimps: current status and treatment methods     

Mondal  Haimanti  Chandrasekaran  Natarajan  Mukherjee  Amitava  Thomas  John 《Aquaculture International》2022,30(1):227-262

Aquaculture is growing post-haste in recent years particularly in the fish and shrimp production. The rapid growth of aquaculture and increasing demand for fish have led to a rapid development of the fish and shrimp industry, resulting in increased production of both fish and shrimps. As a result, there is a greater risk of disease outbreaks. Mass mortalities in aquaculture are primarily due to infectious diseases caused by bacteria, viruses, and fungi. Among them, viral diseases are the most devastating, causing huge loss in the production of both cultured fish and shellfishes. There are several effective methods of treatment for these disease outbreaks. This review focuses on various methods of controlling the viral pathogens using various treatment methods like use of medicinal plants and seaweed extracts, bioactive compounds from actinomycetes, vaccines, probiotic microbes, chemicals, nanoparticles, and green synthesis of nanoparticles.

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Ozone and temperature trends associated with the 11-year solar cycle     

Callis LB  Natarajan M  Nealy JE 《Science (New York, N.Y.)》1979,204(4399):1303-1306

Evidence is presented which suggests that trends in the ozone concentration and stratospheric temperature, reported between the early 1960's and 1976, are to a large extent due to solar ultraviolet flux variability associated with the 11-year solar cycle. Radiative-convective-photochemical simulations of ozone and temperature variations have been made with a solar ultraviolet flux variability model. Results for temperatures and ozone concentrations, when compared with published data, show good agreement.  相似文献   

Bacterial microflora associated with farmed freshwater prawn Macrobrachium rosenbergii (de Man) and the aquaculture environment     

Kuttanappilly V Lalitha  & Poothuvallil K Surendran 《Aquaculture Research》2004,35(7):629-635

The initial counts of bacteria associated with farmed giant freshwater prawn (Macrobrachium rosenbergii de Man) as well as with the water and sediment from two farms located at Kottayam district in Kerala (India) were determined. A total of 367 randomly selected isolates were characterized and identified. Prawn samples yielded mean microbiological counts of 4.92 log CFU g^?1 of shell with muscle and 7.78 log CFU g^?1 of intestine at 30°C. Bacterial numbers in the intestine of prawn were much higher than those in the pond water. Motile and non‐motile aerobic Gram‐negative bacteria together with Enterobacteriaceae accounted for 60–70% of the mesophiles isolated from water and prawn. However, the microorganisms isolated in significant numbers from shell with muscle and intestine of prawn were not recovered from the growing waters. Among Gram‐positive bacteria, Micrococcus, Bacillus, coryneforms, and Arthrobacter were found. Faecal coliforms and enterococci were found in significant numbers in M. rosenbergii. The rearing practices such as feeding and pond fertilization could have influenced the microflora in prawn. The commensal microflora of freshwater prawn included opportunistic pathogens such as Aeromonas spp. and Streptococcus spp. Storage of prawn at elevated temperatures can permit their survival and growth leading to quality loss. Care should be exercised during handling and processing to prevent contamination of edible meat.  相似文献